Pheromone tests for insects

Pheromone tests for insects

Pheromone tests for insects is quite common. For example, the olefin precursor of disparlure — (Z)-2-methyl-7-octadecene — was tested as a mating disruptant in me dium (16 ha) and small (0.01 ha) field plots. In one test, olefin was applied as a mi croencapsulated formulation (NCR) at 62 g/ha to 16-ha plots in a preseason test witl released laboratory reared males.” The test was monitored with traps baited with 48 hr-old virgin females A second pheromones test was conducted against an artificial infestation witl 15 g olefin per hectare on l6-ha plots. A third test was conducted in a natural infesta tion by applying olefin to 16-ha plots at 15 g/ha. Tests were monitored by capture 0 male moths in treated vs. untreated plots or by mating success of females placed it plots. In the first test, reduction of trap catch by 80% or less indicated that the olefir would be of little value for suppression of population thanks to alarm pheromones. The second test substantiatec this conclusion because there was no difference in mating in olefin-treated and un treated plots. Similar data were obtained in the natural population. In another small-plot test (0.01 ha), Cardé et al.” found that disparlure or a mixture of olefin and disparlure reduced the catch of male moths in disparlure—baited trap: vi and that the olefin alone was much less effective. The chemicals were released by evaporation from stainless steel planchets suspended at a height of 1.8 m from strings on 2 I1 4 X 4 checkerboard grid with release stations 3 m apart. 1 Also, in 1978, the tests with NCR microcapsules showed that an application of 50 g disparlure per hectare gave 97% mating reduction whereas 5 g/ha gave only 76% reduction (Table 3). Thus, rate as well as population influenced the reduction of mating. Further information concerning this effect was obtained in small-plot tests in Massachusetts. The objective was to evaluate the technique because it was believed that small plots might permit rapid comparisons between dose levels without excessive expenditure of time and manpower.” This is the true pheromone usage. Learn about Turn Up The Heat pheromone |

Plots of 25 m X 25 m were marked out, and disparlure was applied at various rates to cotton wicks (36 wicks per plot) suspended at 5-m intervals on strings 2 m above the ground. Since the strings were 5 m apart, the wicks formed a regular checkerboard pattern. At the center of the plot was an array of five high-capacity traps, 100 m apart, each baited with (+ )—disparlure. The traps also contained a toxicant that killed moths that entered. Learn about pheromone oils |

Insect catch was determined daily, and the catch in treated plots was compared with that in untreated plots. The tests were carried out in mid- to late July, when moth flight was at its peak, and in early August, when the population was declining. At peak flight, 60 to 300 insects were caught daily in untreated plots, compared with 3 to 20 insects caught daily as the flight decreased. Racemic disparlure was applied to the wicks at loadings that ranged from 1.8 mg to 36 g total per plot. Each treatment was replicated three times. Applications of l g per plot or more reduced male catch by 90%. About 100 mg of disparlure evaporated daily 1 from the 1-g application, equivalent to 250 mg/ha/day. When the, population was reduced thanks to pheromones. Learn more about pheromones at

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